|
Download
protocol
418kb
pdf? |
|
Abstract for PowerPlex® 1.1 System
The PowerPlex® 1.1 System allows the co-amplification and
two-color detection of eight STR loci. In the PowerPlex®
1.1 System, one of the two primers for D16S539, D7S820, D13S317 and D5S818 is labeled with fluorescein (FL), and one primer specific for
CSF1PO, TPOX, TH01 and vWA is labeled with carboxy-tetramethylrhodamine (TMR).
All eight loci are amplified simultaneously in a single tube and analyzed in a
single gel lane. Loci amplified using the PowerPlex® 1.1 System also
can be co-amplified with the GenePrint® Fluorescent Sex
Identification System—Amelogenin (TMR), allowing the detection of nine loci in a
single gel lane.
The PowerPlex® 1.1 System is customized for use with the Hitachi
FMBIO® and FMBIO® II fluorescence imaging
systems. The system provides all of the materials necessary for amplification of
STR regions of purified genomic DNA except for Taq DNA
polymerase. This manual contains protocols for use of the PowerPlex®
1.1 System with the Perkin-Elmer model 480 and GeneAmp® PCR system
9600 thermal cyclers in addition to protocols for separation of amplified
products and detection of separated material. |
