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Abstract for GenePrint^® STR Systems (Silver Stain Detection)

The GenePrint^® STR Systems provide a rapid, non-radioactive method, which can be used to evaluate very small amounts (e.g., 1ng) of human DNA. The protocols detailed in this manual describe the use of silver staining to detect the presence of amplified STR products following their separation by denaturing polyacrylamide gel electrophoresis.

The GenePrint^® STR Systems provide all of the materials required to perform 100 or 400 amplification reactions except for Taq DNA polymerase and sample DNA. This manual describes methods we have evaluated and recommend for sample preparation, amplification of sample, separation of amplified products and silver detection of separated material. All of the GenePrint^® STR Systems can be amplified using either the Perkin-Elmer model 480 or 9600 thermal cyclers, but slight differences in yield or balance between loci might be observed if the system is not first optimized on that particular thermal cycler. This manual provides a number of cycling protocol options so that excellent results can be obtained regardless of the thermal cycler used.

Protocols
Index

Part# TMD004
Printed in USA. Revised 7/06
Instructions for Use of Products DC1191, DC4001, DC4011, DC4021, DC4031, DC4041, DC4051, DC4061, DC4071, DC4080, DC4081, DC6000, DC6001, DC6030, DC6031, DC6450, DC6451, DG2141 & DG2101: Request this protocol.

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