Abstract for HaloTag® Protein Purification System Technical Manual
Part# TM312
Printed in USA Revised 10/09.
Instructions for Use of Products G6270
& G6280.
The HaloTag® Protein Purification System is based on a unique tag, the HaloTag® protein, which allows covalent, efficient and specific capture of the
proteins expressed in E. coli as N-terminal HaloTag® fusion proteins. The HaloTag® protein is a mutated hydrolase that covalently attaches to the HaloLink™ Resin via an immobilized chloroalkane. The HaloTag® Protein
Purification System allows stringent washing because of the covalent attachment
of the HaloTag® protein to the resin. TEV Protease cleaves the target protein from
the HaloTag® protein which is bound to the HaloLink™ Resin. Next, the TEV
Protease, which has an N-terminal (HQ)3 tag, is removed from the protein of
interest using the HisLink™ Resin, and the purified protein of interest is
recovered.
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