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Abstract for StemElite™ ID System
Cell line misidentification and cross-contamination have
become important concerns for researchers. For example, stem cell
lines can become contaminated by the mouse cell line making up the
feeder layers on which stem cells are propagated. Genetic profiling
can be used as a tool for stem cell line quality assurance, and
human stem cell lines can be authenticated using short tandem repeat
(STR) loci. STR loci consist of short repetitive sequence elements
3–7 base pairs in length. These repeats are well distributed
throughout the human genome and are a rich source of highly
polymorphic markers, which can be detected using polymerase chain reaction.
To provide a powerful and complete system for cell line
identification we developed the StemElite™ ID System, which contains all reagents required for successful identification and authentication of human stem cell lines and detection of cell line contaminants. Additionally we have incorporated a sensitive marker that specifically detects the presence of DNA from the mouse (Mus musculus). This locus has been developed to amplify with the existing human loci in the primer set provided with this system.
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