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Abstract for TNT® T7 Insect Cell Extract System
The TNT® T7 Insect Cell Extract Protein Expression System is a
convenient, quick, single-tube, coupled transcription/translation
system for cell-free expression of proteins. It uses the TNT®
technology to allow both transcription and translation to occur in a
single reaction. The protein synthesis reactions are initiated by
adding a DNA template, eliminating the need for the time-consuming
process of in vitro RNA synthesis and increasing protein yield.
The extract is made from the commonly used Spodoptera frugiperda
Sf21 cell line. All components necessary for the
transcription/translation reaction have been added to the extract
and are present in the TNT® T7 ICE Master Mix. To begin protein
synthesis, just add the DNA template. This format simplifies and
reduces the time required to set up reactions. Reactions are
incubated at 28–30°C and are complete within 4 hours.
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