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Abstract for Protease-Glo™ Assay
The Protease-Glo™ Assay is a novel method to detect and measure
protease activities using a genetically engineered firefly (Photinus pyralis)
luciferase. The assay uses a circularly permuted firefly luciferase, the
GloSensor™-10F protein, with a protease recognition site as the protease
substrate. This assay system allows rapid generation of protease substrates through
molecular cloning and coupled transcription/translation cell-free expression, thus
enabling the facile evaluation of protease function. |
