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Abstract for Plexor® HY System for the Corbett Rotor-Gene 6000 Series Detection System

The Plexor® System is a novel real-time PCR system for quantitation of total human (autosomal) and human male (Y) DNA within a sample. This Technical Manual describes the Plexor® HY System and provides instructions for use with the Corbett Rotor-Gene 6000 Series Detection System, including reaction setup, real-time PCR instrument programming and data analysis using the Plexor® Analysis Software.

The Plexor® technology takes advantage of the specific interaction between two modified nucleotides to achieve quantitative PCR analysis. One of the PCR primers contains a modified nucleotide (iso-dC) linked to a fluorescent label at the 5′ end. The second PCR primer is unlabeled. The reaction mix includes deoxynucleotides and iso-dGTP modified with the quencher dabcyl. Dabcyl-iso-dGTP is incorporated opposite the iso-dC residue in the primer. The incorporation of the dabcyl-iso-dGTP at this position results in quenching of the fluorescent dye on the complementary strand and a reduction in fluorescence, which allows quantitation during amplification.


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Part# TM299
Printed in USA. Revised 1/09.
Instructions for Use of Products DC1000 & DC1001: Request this protocol.

Printed publications disclaimer.