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Abstract for Plexor HY System for the Corbett Rotor-Gene 6000
Series Detection System
The Plexor® System is a novel real-time PCR system
for quantitation of total human (autosomal) and human male (Y) DNA
within a sample. This Technical Manual describes the Plexor® HY
System and provides instructions for use with the Corbett Rotor-Gene
6000 Series Detection System, including reaction set-up, real-time
PCR instrument programming and data analysis using the Plexor®
Analysis Software.
The Plexor® technology takes advantage of the specific
interaction between two modified nucleotides to achieve quantitative PCR analysis. One of the PCR primers contains a modified nucleotide
(iso-dC) linked to a fluorescent label at the 5' end. The second PCR
primer is unlabeled. The reaction mix includes deoxynucleotides and
iso-dGTP modified with the quencher dabcyl. Dabcyl-iso-dGTP is
incorporated opposite the iso-dC residue in the primer. The
incorporation of the dabcyl-iso-dGTP at this position results in
quenching of the fluorescent dye on the complimentary strand and a
reduction in fluorescence, which allows quantitation during
amplification. |
