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Abstract for ONE-Glo™ Luciferase Assay System
High- and ultrahigh-throughput quantitation of luciferase
expression in mammalian cells requires sensitive reagents. The
ONE-Glo™ Luciferase Assay System employs new assay chemistry
to provide a robust, homogeneous assay. A new luciferase substrate
has enabled Promega to create a novel reagent that is more robust
and stable when reconstituted, and less aromatic than
standard reagents created with luciferin.
The ONE-Glo™ Luciferase Assay Buffer and ONE-Glo™ Luciferase
Assay Substrate, provided with this system, are combined to make the
ONE-Glo™ Reagent. |