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Abstract for Plexor™ Systems Instrument Setup and Data Analysis
for the Roche LightCycler® 480 System
The Plexor™ qPCR and qRT-PCR Systems are compatible with a
variety of real-time PCR instruments. Data from these instruments
can be analyzed with one dedicated software program, the Plexor™
Analysis Software. This manual includes instructions and thermal cycling
conditions specific for use of the Plexor™ qPCR
System, Plexor™ Two-Step qRT-PCR System and Plexor™ One-Step qRT-PCR
System with the Roche LightCycler® 480 System. Instructions
are included for instrument setup, data transfer from the instrument to the
Plexor™ Analysis Software and data analysis.
This Technical Manual is available in pdf format. The entire
document may be downloaded by selecting the icon to the left.
Because the entire document is a large file, the manual subsections
are also provided below for ease of access to the specific parts of
the document.
Table of Contents
- Description, and II.,
Plate Preparation and Amplification (pdf 85kb)
- Generating Color Compensation Files (pdf 3365kb)
-
Instrument Setup and
Thermal Cycling for qPCR and Two-Step qRT-PCR (pdf 1847kb)
- Instrument Setup and
Thermal Cycling for One-Step qRT-PCR (pdf 1792kb)
- Instrument Setup and
Thermal Cycling for Genotyping (SNP) Assays (pdf 1811kb)
- Data Export
from the Roche LightCycler® Data Analysis Software and
-
Data Import into the Plexor™ Analysis
Software (pdf 1341kb)
-
Data Analysis With the Plexor™ Analysis Software (pdf
1657kb)
-
Troubleshooting (pdf
215kb)
-
Appendix (Plexor™
Analysis Software Operating System Compatibility, Plexor™
Analysis Software Installation, Advanced Options, Manual
Baseline Adjustments, Icon Definitions, Amplification Efficiency
Calculations, Reference) (pdf 1113kb)
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