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Abstract for ChipShot™ Indirect Labeling and Clean-Up System
The ChipShot™ Indirect Labeling and Clean-Up System generates
fluorescent
cDNAs by synthesizing an aminoallyl cDNA and then conjugating the
CyDye™
NHS ester to the cDNA. Either total RNA or poly(A)+ mRNA can be used
with
these systems. Resulting cDNAs are labeled to provide efficient
microarray
hybridization results. The ChipShot™ Indirect Labeling System
includes a
human Total RNA Positive Control to evaluate the performance of the synthesis, labeling and clean-up procedures. The ChipShot™ Membrane
Clean-Up System produces highly purified, labeled cDNAs. Amplification of
RNA is
not required. All reagents are specifically optimized for microarray applications.
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