Abstract for ChipShot™ Indirect Labeling and Clean-Up System

Part# TB287
Printed in USA. Revised 3/09.
Instructions for Use of Product Z4000.

The ChipShot™ Indirect Labeling and Clean-Up System generates fluorescent cDNAs by synthesizing an aminoallyl cDNA and then conjugating the CyDye™ NHS ester to the cDNA. Either total RNA or poly(A)+ mRNA can be used with these systems. Resulting cDNAs are labeled to provide efficient microarray hybridization results. The ChipShot™ Indirect Labeling System includes a human Total RNA Positive Control to evaluate the performance of the synthesis, labeling and clean-up procedures. The ChipShot™ Membrane Clean-Up System produces highly purified, labeled cDNAs. Amplification of RNA is not required. All reagents are specifically optimized for microarray applications.