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Abstract for ChipShot™ Direct Labeling and Clean-Up System
The ChipShot™ Direct Labeling and Clean-Up System generates
fluorescent
cDNAs by direct labeling using Cy®3 and Cy®5 dCTP. Either total RNA or
poly(A)+ mRNA can be used with these systems. Resulting cDNAs are
labeled
to provide efficient microarray hybridization results. The ChipShot™
Direct
Labeling System includes a human Total RNA Positive Control to
evaluate the
performance of the labeling and labeling clean-up procedures. The
ChipShot™
Membrane Clean-Up System produces highly purified, labeled cDNAs
that are
essentially free of unincorporated nucleotides. Amplification of RNA
is not
required. All reagents are specifically optimized for microarray
applications.
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