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Abstract for TNT® SP6 High-Yield Protein Expression System
The TNT® SP6 High-Yield Protein Expression System is a
convenient, quick, single-tube, coupled transcription/translation
system designed to express up to 100µg/ml of protein. This cell-free
expression system is prepared from an optimized wheat germ extract
and contains all the components (tRNA, ribosomes, amino acids,
polymerase and translation initiation, elongation and termination
factors) necessary for protein synthesis directly from DNA
templates.
The TNT® SP6 High-Yield Protein Expression System expresses genes
cloned downstream of an SP6 RNA polymerase promoter.
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