|
Download
protocol
2,312kb
pdf? |
|
Abstract for Plexor™ Systems Instrument Setup and Data Analysis
for the Roche LightCycler® 2.0 and LightCycler®
1.5 Systems using LightCycler® Software Version 4.0
The Plexor™ qPCR and qRT-PCR Systems are compatible with a
variety of real-time PCR instruments. Data from these instruments
can be analyzed with one dedicated software program, the Plexor™
Analysis Software. This manual includes instructions and thermal cycling
conditions specific for use of the Plexor™ qPCR
System, Plexor™ Two-Step qRT-PCR System and Plexor™ One-Step qRT-PCR
System with the Roche LightCycler® 2.0 System. Instructions
are included for instrument setup, data transfer from the instrument to the
Plexor™ Analysis Software and data analysis.
This Technical Manual is available in pdf format. The entire
document may be downloaded by selecting the icon to the left.
Because the entire document is a large file, the manual subsections
are also provided below for ease of access to the specific parts of
the document that you may need.
Table of Contents
- Description, and II.,
Capillary Preparation and Amplification (pdf 52kb)
-
Generating the Color Compensation Object (172 pdf )
-
Instrument Setup and
Thermal Cycling for qPCR and Two-Step qRT-PCR (pdf 268kb)
- Instrument Setup and
Thermal Cycling for One-Step qRT-PCR (pdf 289kb)
- Instrument Setup and
Thermal Cycling for Genotyping (SNP) Assays (pdf 533kb)
- Data Export
from the Roche LightCycler® Data Analysis Software and
VIII., Data Import into the Plexor™ Analysis
Software (pdf 221b)
- Data Analysis With the
Plexor™ Analysis Software (pdf 598kb)
- Troubleshooting
and Appendix (Plexor™
Analysis Software Operating System Compatibility, Plexor™
Analysis Software Installation, Advanced Options, Manual
Baseline Adjustments, Icon Definitions, Amplification Efficiency
Calculations, Reference) (pdf 302kb)
|
