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Abstract for Plexor™ Systems Instrument Setup and Data Analysis
for the DNA Engine Opticon® 2 Real-Time PCR Detection
System
The Plexor™ qPCR and qRT-PCR Systems are compatible with a
variety of real-time PCR instruments. Data from these instruments
can be analyzed with one dedicated software program, the Plexor™
Analysis Software. This manual includes
instructions and thermal cycling conditions specific for use of the
Plexor™ qPCR
System, Plexor™ Two-Step qRT-PCR System and Plexor™ One-Step qRT-PCR
System with the DNA Engine Opticon® 2 Real-Time PCR Detection
System. Instructions are included for instrument setup, data
transfer from the instrument to the Plexor™ Analysis Software and
data analysis.
This Technical Manual is available in pdf format. The entire
document may be downloaded by selecting the icon to the left.
Because the entire document is a large file, the manual subsections
are also provided below for ease of access to the specific parts of
the document that you may need.
Table of Contents
- Description, and II., Plate
Preparation and Amplification (pdf 90kb)
-
Instrument Setup and Thermal
Cycling for qPCR and Two-Step qRT-PCR (pdf 2346kb)
- Instrument Setup and
Thermal Cycling for One-Step qRT-PCR (pdf 1709kb)
- Instrument Setup and Thermal
Cycling for Genotyping (SNP) Assays (pdf 1919kb)
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Data Export from the Opticon Monitor Software and VII., Data Import into
the Plexor™ Analysis Software (pdf 1122kb)
- Data Analysis With the
Plexor™ Analysis Software (pdf 1689kb)
- Troubleshooting (pdf 115kb)
- Appendix (Operating
System Compatibility, Analysis Software
Installation, Advanced Options, Manual Baseline Adjustments,
Icon Definitions, Amplification Efficiency Calculations, Special
Considerations for Importing Comma-Delimited Data files,
Reference) (pdf 578kb)
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