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Abstract for Plexor™ Systems Instrument Setup and Data Analysis
for the Bio-Rad iCycler iQ™ Real-Time PCR Detection System
The Plexor™ qPCR and qRT-PCR Systems are compatible with a
variety of real-time PCR instruments. Data from these instruments
can be analyzed with one dedicated software program, the Plexor™
Analysis Software. This manual includes instructions and thermal cycling
conditions specific for use of the Plexor™ qPCR
System, Plexor™ Two-Step qRT-PCR System and Plexor™ One-Step qRT-PCR
System with the Bio-Rad iCycler iQ™ Real-Time PCR Detection System.
Instructions are included for instrument setup, data transfer from the
instrument to the Plexor™ Analysis Software and data analysis.
This Technical Manual is available in pdf format. The entire
document may be downloaded by selecting the icon to the left.
Because the entire document is a large file, the manual subsections
are also provided below for ease of access to the specific parts of the
document that you may need.
Table of Contents
- Description, and
II., Plate Preparation and Amplification (pdf 85kb)
- Instrument Setup and
Thermal Cycling for qPCR and Two-Step qRT-PCR (pdf 877kb)
- Instrument Setup and
Thermal Cycling for One-Step qRT-PCR (pdf 868kb)
- Instrument Setup and
Thermal Cycling for Genotyping (SNP) Assays (pdf 873kb)
- Data Export from the
iCycler™ and Import into the Plexor™ Analysis Software (pdf
1628kb)
- Data Analysis With
the Plexor™ Analysis Software (pdf 1529kb)
- Troubleshooting (pdf
103kb)
- Appendix (Operating
System Compatibility, Analysis Software Installation, Advanced
Options, Manual Baseline Adjustments, Icon Definitions,
Amplification Efficiency Calculations, Special Considerations
for Importing Comma-Delimited Data files, Reference (pdf 537kb)
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