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Abstract for Plexor® Systems Instrument Setup and Data Analysis
for the Roche LightCycler® 1.0 and LightCycler® 1.5 Systems
using LightCycler® Software Version 3.5
The Plexor® qPCR and qRT-PCR Systems are compatible with a
variety of real-time PCR instruments. Data from these instruments
can be analyzed with one dedicated software program, the Plexor®
Analysis Software. This manual includes instructions and thermal cycling
conditions specific for use of the Plexor™ qPCR
System, Plexor® Two-Step qRT-PCR System and Plexor® One-Step qRT-PCR
System with the Roche LightCycler® 1.0 and 1.5 Systems
using the LightCycler® Software version 3.5.
Instructions are included for instrument setup, data
transfer from the instrument to the Plexor®
Analysis Software and
data analysis.ysis.
This Technical Manual is available in pdf format. The entire
document may be downloaded by selecting the icon to the left.
Because the entire document is a large file, the manual subsections
are also provided
below for ease of access to the specific parts of the document that
you may need.
Table of Contents
- Description, and II.,
Capillary Preparation and Amplification (pdf 94kb)
- Generating the Color Compensation
File (pdf 382kb)
- Instrument Setup and
Thermal Cycling for qPCR and Two-Step qRT-PCR (pdf 258kb)
- Instrument Setup and
Thermal Cycling for One-Step qRT-PCR (pdf 248kb)
- Instrument Setup and
Thermal Cycling for Genotyping (SNP) Assays (pdf 246kb)
- Data Export from the
Roche LightCycler® Data Analysis Software (pdf
154kb)
- Data Import
into the Plexor® Analysis Software (pdf 331)
- Data Analysis With the
Plexor® Analysis Software (pdf 599kb)
- Troubleshooting (pdf
124kb)
- Appendix (Operating
System Compatibility, Analysis Software
Installation, Advanced Options, Manual Baseline Adjustments,
Icon Definitions, Amplification Efficiency Calculations, Special
Considerations for Importing Comma-Delimited Data files,
Reference) (pdf 391kb)
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