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Abstract for Plexor™ Systems Instrument Setup and Data Analysis
for the Applied Biosystems 7900HT Real-Time PCR System
The Plexor™ qPCR and qRT-PCR Systems are compatible with a
variety of real-time PCR instruments. Data from these instruments
can be analyzed with one dedicated software program, the Plexor™
Analysis Software. This manual includes instructions and thermal cycling
conditions specific for use of the Plexor™ qPCR
System, Plexor™ Two-Step qRT-PCR System and Plexor™ One-Step qRT-PCR
System with the Applied Biosystems 7900HT Real-Time PCR System.
Instructions are included for instrument setup, data
transfer from the instrument to the Plexor™ Analysis Software and
data analysis.
This Technical Manual is available in pdf format. The entire
document may be downloaded by selecting the icon to the left.
Because the entire document is a large file, the manual subsections
are also provided
below for ease of access to the specific parts of the document that
you may need.
Table of Contents
- Description, and
II., Plate Preparation and Amplification (pdf 65kb)
- Instrument Setup and
Thermal Cycling for qPCR and Two-Step qRT-PCR (pdf 727kb)
- Instrument Setup and
Thermal Cycling for One-Step qRT-PCR (pdf 744kb)
- Instrument Setup and
Thermal Cycling for Genotyping (SNP) Assays (pdf 759kb)
- Data Export from
the Applied Biosystems 7900HT Real-Time PCR System and VII., Data
Import into the Plexor™ Analysis Software (pdf 524kb)
- Data Analysis With
the Plexor™ Analysis Software (pdf 1621kb)
- Troubleshooting (pdf
101kb)
- Appendix (Operating
System Compatibility, Analysis Software Installation, Advanced
Options, Manual Baseline Adjustments, Icon Definitions,
Amplification Efficiency Calculations, Special Considerations
for Importing Comma-Delimited Data files, Reference (pdf 573kb)
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