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Abstract for Plexor™ Systems Instrument Setup and Data Analysis for the ABI PRISM^® 7700 Sequence Detection System

The Plexor™ qPCR and qRT-PCR Systems are compatible with a variety of real-time PCR instruments. Data from these instruments can be analyzed with one dedicated software program, the Plexor™ Analysis Software. This manual includes instructions and thermal cycling conditions specific for use of the Plexor™ qPCR System, Plexor™ Two-Step qRT-PCR System and Plexor™ One-Step qRT-PCR System with the ABI PRISM^® 7700 Sequence Detection System. Instructions are included for instrument setup, data transfer from the instrument to the Plexor™ Analysis Software and data analysis.

This Technical Manual is available in pdf format. The entire document may be downloaded by selecting the icon to the left. Because the entire document is a large file, the manual subsections are also provided below for ease of access to the specific parts of the document that you may need.

Table of Contents

1. Description, and II., Plate Preparation and Amplification (pdf 88kb)

3. Instrument Setup and Thermal Cycling for qPCR and Two-Step qRT-PCR (pdf 368kb)
4. Instrument Setup and Thermal Cycling for One-Step qRT-PCR (pdf 369kb)
5. Instrument Setup and Thermal Cycling for Genotyping (SNP) Assays (pdf 376kb)
6. Data Export from the ABI PRISM^® 7700 Sequence Detection System and VII., Data Import into the Plexor™ Analysis Software (pdf 587kb)

8. Data Analysis With the Plexor™ Analysis Software (pdf 1700kb)
9. Troubleshooting (pdf 117kb)
10. Appendix (Operating System Compatibility, Analysis Software Installation, Advanced Options, Manual Baseline Adjustments, Icon Definitions, Amplification Efficiency Calculations, Reference) (pdf 591kb)

Protocols
Index

Part# TM267
Printed in USA 9/05
Instructions for Use of Products A4011, A4021, A4051 (available for purchase outside of Europe only) and
A4031, A4041 & A4061 (available for purchase only in Europe).

Printed publications disclaimer.