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Abstract for Plexor™ Systems Instrument Setup and Data Analysis
for the ABI PRISM® 7000 Sequence Detection System
The Plexor™ qPCR and qRT-PCR Systems are compatible with a
variety of real-time PCR instruments. Data from these instruments
can be analyzed with one dedicated software program, the Plexor™
Analysis Software. This manual includes
instructions and thermal cycling conditions specific for use of the
Plexor™ qPCR
System, Plexor™ Two-Step qRT-PCR System and Plexor™ One-Step qRT-PCR
System with the ABI PRISM® 7000 Sequence Detection System.
Instructions are included for instrument setup, data
transfer from the instrument to the Plexor™ Analysis Software and
data analysis.
This Technical Manual is available in pdf format. The entire
document may be downloaded by selecting the icon to the left.
Because the entire document is a large file, the manual subsections
are also provided
below for ease of access to the specific parts of the document that
you may need.
Table of Contents
- Description, and
II., Plate Preparation and Amplification (pdf 74kb)
- Instrument Setup and
Thermal Cycling for qPCR and Two-Step qRT-PCR (pdf 555kb)
- Instrument Setup and
Thermal Cycling for One-Step qRT-PCR (pdf 567kb)
- Instrument Setup and
Thermal Cycling for Genotyping (SNP) Assays (pdf 533kb)
- Data Export from
the ABI PRISM® 7000 Sequence Detection System
Software and VII., Data Import into the Plexor™ Analysis
Software (pdf 548kb)
- Data Analysis With the
Plexor™ Analysis Software (pdf 1720kb)
- Troubleshooting (pdf
134kb)
- Appendix (Operating
System Compatibility, Analysis Software
Installation, Advanced Options, Manual Baseline Adjustments,
Icon Definitions, Amplification Efficiency Calculations, Special
Considerations for Importing Comma-Delimited Data files,
Programming the ABI PRISM® 7000 Sequence Detection
System Running SDS Software Version 1.0, Reference) (pdf 550kb)
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