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Abstract for Plexor™ Systems Instrument Setup and Data Analysis
for the Applied Biosystems 7300 and 7500 Real-Time PCR Systems
The Plexor™ qPCR and qRT-PCR Systems are compatible with a
variety of real-time PCR instruments. Data from these instruments
can be analyzed with one dedicated software program, the Plexor™
Analysis Software. This manual includes instructions and thermal cycling
conditions specific for use of the Plexor™ qPCR System, Plexor™
Two-Step qRT-PCR System and Plexor™ One-Step qRT-PCR
System with the Applied Biosystems 7300 and 7500 Real Time PCR
Systems. Instructions are included for instrument setup, data
transfer from the instrument to the Plexor™ Analysis Software and
data analysis.
This Technical Manual is available in pdf format. The entire
document may be downloaded by selecting the icon to the left.
Because the entire document is a large file, the manual subsections
are also provided below for ease of access to the specific parts of
the document that you may need.
Table of Contents
- Description, and II.,
Plate Preparation and Amplification (pdf 64kb)
- Instrument Setup and
Thermal Cycling for qPCR and Two-Step qRT-PCR (pdf 753kb)
- Instrument Setup and
Thermal Cycling for One-Step qRT-PCR (pdf 599kb)
- Instrument Setup and
Thermal Cycling for Genotyping (SNP) Assays (pdf 599kb)
- Data Export from the
Applied Biosystems 7300 and 7500 Real-Time PCR Systems and
VII., Data Import into the Plexor™ Analysis
Software (pdf 680kb)
- Data Analysis With the
Plexor™ Analysis Software (pdf 1901kb)
- Troubleshooting (pdf
100kb)
- Appendix (Operating
System Compatibility, Analysis Software
Installation, Advanced Options, Manual Baseline Adjustments,
Icon Definitions, Amplification Efficiency Calculations, Special
Considerations for Importing Comma-Delimited Data files,
Reference) (pdf 613kb)
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