Abstract for Plexor^® Two-Step qRT-PCR System Technical Manual

Part# TM264
Printed in USA. Revised 11/09.
Instructions for Use of Products A4051 (available for purchase outside of Europe only) & A4061 (available for purchase in Europe only).

The Plexor^® qPCR System is a novel real-time PCR system for the quantitation of specific sequences within an RNA sample. This system uses ImProm-II™ Reverse Transcriptase for cDNA synthesis and the Plexor^® qPCR technology for quantitation of any type of RNA sample, including total cellular RNA, poly(A)+ RNA or viral RNA. With this system, a separate reverse transcription reaction is assembled, and an aliquot of the resulting cDNA is added to an amplification reaction. This allows multiple amplifications to be performed using the same reverse transcription reaction.