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Abstract for GeneClip™ U1 Hairpin Cloning Systems
The GeneClip™ U1 Hairpin Cloning Systems consist of linearized plasmids
designed for easy and fast cloning of hairpin target sequences to allow expression of
siRNA target sequences in human cells. The plasmids contain the human U1 promoter,
which allows transcription of the hairpin target sequences and generation of hairpin siRNA in vivo.
The GeneClip™ U1 Hairpin Cloning System—Basic is intended for
transient suppression of the gene of interest. The GeneClip™ U1
Hairpin Cloning System—hMGFP enables easy determination of
transfection efficiency and allows selection of transfected cells by
fluorescence-activated cell sorting (FACS®). The other
GeneClip™ U1 Hairpin Cloning Systems offer antibiotic marker options
(neomycin, hygromycin or puromycin) for selecting stably transfected
eukaryotic cells so that experimental results do not depend on
transfection efficiency.
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