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Abstract for MSI Analysis System, Version 1.2
The MSI Analysis System, Version 1.2, is a fluorescent PCR-based
assay for detection of microsatellite instability. Typically, MSI
analysis involves comparing allelic profiles of microsatellite
markers generated by amplification of DNA from matching normal and
tumor samples. Alleles that are present in the tumor sample but not
found in the corresponding normal samples indicate MSI. The MSI
Analysis System includes fluorescently labeled primers for
co-amplification of seven markers including five mononucleotide
repeat markers (BAT-25, BAT-26, NR-21, NR-24 and MONO-27) and two
pentanucleotide repeat markers
(Penta C and Penta D). The mononucleotide markers are used for MSI
determination, and the pentanucleotide markers are used to detect
potential sample mixups and/or contamination. Internal lane size
standards are added to the PCR samples to assure accurate sizing of
alleles and to adjust for run-to-run variation. The PCR products are
separated by capillary electrophoresis using an ABI PRISM®
310, 3100 or 3100-Avant Genetic Analyzer or Applied Biosystems 3130
or 3130xl Genetic Analyzer. The output data maybe analyzed with
GeneMapper® Analysis Software to determine MSI status of
tumor samples.
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