|
Download
protocol
613kb
pdf? |
|
Abstract for Flexi® Vector Systems
The Flexi® Vector Systems use two rare-cutting restriction
enzymes, Sgf I and Pme I, in a simple, yet powerful, directional
cloning method for protein-coding sequences. These systems provide a rapid,
efficient and high-fidelity way to transfer these sequences between a variety of
Flexi® Vectors. The Flexi® Vectors contain various
expression or peptide tag options to enable expression of native or fusion proteins
to study protein structure and function, as well as protein-protein interactions.
All Flexi® Vectors carry the lethal barnase gene, which is replaced
by the DNA fragment of interest and acts as a positive selection for successful
ligation of the insert.
(View complete list of Flexi® Vectors.)
|
