Abstract for ImProm-II™ Reverse Transcription System
Technical Manual
Part# TM236
Printed in USA. Revised 8/09.
Instructions for Use of Product A3800.
The ImProm-II™ Reverse Transcription System is a convenient kit that
includes a reverse transcriptase and an optimized set of
reagents designed for efficient synthesis of first-strand cDNA in preparation
for PCR amplification. The components of the ImProm-II™ Reverse Transcription
System can be used to reverse transcribe RNA templates starting with either total
RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimized reaction buffer and
powerful reverse transcriptase provided in the ImProm-II™ Reverse
Transcription System enable robust, full-length cDNA synthesis for
the reproducible analysis of rare or long messages. These conditions
have been developed for cDNA synthesis or for easy
transition to gene-specific target amplification.
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