|
Download
protocol
302kb
pdf? |
|
Abstract for the Rabbit Reticulocyte Lysate System
Rabbit Reticulocyte Lysate Translation Systems are utilized in the
identification of mRNA species, the characterization of their protein products
and the investigation of transcriptional and translational control. Rabbit
Reticulocyte Lysate is prepared from New Zealand white rabbits using a standard
protocol that ensures reliable and consistent reticulocyte production in
each lot. The reticulocytes are purified to remove contaminating cells, which
could otherwise alter the translational properties of the final extract. After
the reticulocytes are lysed, the extract is treated with micrococcal nuclease to
destroy endogenous mRNA and thus reduce background translation to a minimum. The
lysate contains the cellular components necessary for protein synthesis (tRNA,
ribosomes, amino acids, initiation, elongation and termination factors).
Rabbit Reticulocyte Lysate may contain a variety of post-translational
processing activities, including acetylation, isoprenylation and some
phosphorylation activity. Processing events such as signal peptide cleavage and
core glycosylation can be examined by adding Canine Pancreatic Microsomal
Membranes (Cat.# Y4041) to a standard translation reaction.
|
