Abstract for Wheat Germ Extract Plus Technical Manual

Part# TM066
Printed in USA. Revised 8/09.
Instructions for Use of Products L3250 & L3251.

Wheat Germ Extract Plus is a highly efficient, single-tube protein expression system designed to give yields in the range of 10-80µg/ml. The extract contains all the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, and initiation, elongation and termination factors). Potassium acetate and magnesium acetate are also included at concentrations that enhance translation of a wide range of mRNAs. Protein synthesis is initiated by addition of the appropriate mRNA template, and the reaction is incubated for 2 hours at 25ºC. Synthesized proteins can be analyzed by SDS-PAGE or used directly in numerous applications. There is no requirement for dialysis or specific incubation equipment.