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Abstract for Wheat Germ Extract Plus Technical Manual
Wheat Germ Extract Plus is a highly efficient, single-tube
protein expression system
designed to give yields in the range of 10-80μg/ml. The extract
contains all the
cellular components necessary for protein synthesis (tRNA, ribosomes,
amino acids,
and initiation, elongation and termination factors). Potassium
acetate and
magnesium acetate are also included at concentrations that enhance
translation of a
wide range of mRNAs. Protein synthesis is initiated by addition of
the appropriate
mRNA template, and the reaction is incubated for 2 hours at 25ºC.
Synthesized proteins can be analyzed by SDS-PAGE or used directly in
numerous
applications. There is no requirement for dialysis or specific
incubation equipment. |
