|
Download
protocol
331kb
pdf? |
|
Abstract for ViviRen™ Live Cell Substrate
ViviRen™ Live Cell Substrate is a proprietary compound
that generates Renilla luciferase luminescence from live
cells under normal growth conditions. The substrate produces
Renilla luciferase luminescence that is 3- to 5-fold brighter
than coelenterazine in live cells. Low autoluminescence from the
substrate permits signal-to-background ratios that are 100-fold
higher than those for coelenterazine. ViviRen™ Substrate can be used
in a variety of cell analysis techniques including reporter gene
assays, RNA interference and bioluminescence resonance energy
transfer (BRET), because the substrate permits real-time measurement
in multiwell plates.
|
