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Abstract for Dual-Glo™ Luciferase Assay System
The Dual-Glo™ Luciferase Assay System is designed to allow high-throughput
analysis of mammalian cells containing genes for firefly and Renilla
luciferases,
grown in 96- or 384-well plates. The Dual-Glo™ Luciferase Reagent can be added
directly to cells in growth medium without washing or preconditioning. This
reagent induces cell lysis and acts as a substrate for firefly luciferase,
producing a stable luminescent signal that can be read over a period of two
hours, with >60% of the luminescent signal retained. Addition of Dual-Glo™
Stop & Glo® Reagent quenches the luminescence from the firefly reaction by
at least 10,000-fold and provides a substrate for Renilla luciferase in a
reaction that can also be read within 2 hours (with similar signal
retention). The Dual-Glo™ Luciferase Assay System is designed to work in
growth media commonly used for mammalian cells with or without added serum.
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