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Abstract for Renilla Luciferase Assay System
The Renilla Luciferase Assay System was developed for
reporter quantitation in mammalian cells. Genetic reporters are used commonly in
cell biology to study gene expression and other cellular events coupled to gene
expression, such as receptor activity, intracellular signal transduction, mRNA
processing, protein folding and protein-protein interactions. Renilla
luciferase, a monomeric 36kDa protein, catalyzes coelenterate-luciferin (coelenterazine)
oxidation to produce light. Post-translational modification is not required for
its activity, and the enzyme may function as a genetic reporter immediately
following translation.
The Renilla Luciferase Assay System is designed to provide a
fast and sensitive method of detecting sea pansy (Renilla
reniformis) luciferase. This system is a convenient alternative to firefly (Photinus
pyralis) reporter systems and is designed to yield reliable, linear results
for a concentration range over seven orders of magnitude. The Renilla
Luciferase Assay System has been formulated with a proprietary composition that
significantly reduces the coelenterazine autoluminescence commonly seen with
other reagents. The result is an assay system that is more sensitive than
published methods by several orders of magnitude. This sensitivity is comparable
to that seen with the firefly Luciferase Assay System. The Renilla
Luciferase Assay System enables measurement of Renilla luciferase
activity using either the wildtype or new synthetic Renilla luciferase
as a primary reporter to study transcriptional regulation or as a co-reporter
for normalization of experimental variations such as differences in transfection
efficiencies. |
