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Abstract for the READIT® SNP Genotyping System
The READIT® SNP Genotyping System is a nonradioactive, gel-free method for
identifying specific DNA sequences and genotyping DNA samples at known alleles.
This molecular technology is capable of analyzing a variety of sequence
variations, including single nucleotide polymorphisms (SNPs), insertions,
deletions and chromosomal translocations. The READIT® System is also capable
of multiplexed PCR and RT-PCR product detection in a high-throughput,
arrayed-plate format. To identify the unique sequence of interest, interrogation
probes are hybridized to a purified, PCR-generated DNA template. Only
interrogation probes with perfect homology at the 3′-end are appropriate
substrates for the READIT® Assay. For example, a bi-allelic polymorphism
requires two interrogation probes that differ only at their 3′-terminus to form
perfect hybrids with only one of the two alleles.
The READIT® assay can be performed manually or automated on the
Biomek® 2000 instrument. Both manual and automated protocols are
included in this Technical Manual.
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