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Abstract for Bright-Glo™ Luciferase Assay System
Ultrahigh-throughput quantitation of luciferase expression in mammalian cells
requires highly sensitive reagents that can adapt to continuous process robotic
systems. The Bright-Glo™ Luciferase Assay System is designed
specifically to meet the needs of continuous process systems by providing
robust, homogeneous assay chemistry that achieves high assay sensitivity and
approximately half-hour signal half-life without prior sample processing. These
attributes also benefit scientists using fewer samples who still require high
sensitivity and ease of use.
The Bright-Glo™ Luciferase Assay System has been designed for use
with the following culture media containing 0-10% serum: RPMI 1640, MEM-alpha,
DMEM and Ham’s F12. The signal half-life under these conditions will generally
exceed 25 minutes at 22°C and is independent of enzyme concentration where the
luciferase concentration is <10-9M. Other media/sera combinations
can also be used, but experimental verification of assay performance is
recommended in these cases. |
