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Abstract for SV Total RNA Isolation System
The purity and integrity of RNA isolated from tissue or cultured cells are
critical for its effective use in applications such as reverse transcription PCR
(RT-PCR), real-time PCR, RNase protection assays, Northern blot analysis, oligo(dT) selection of poly(A)+ RNA, in vitro translation and
microarray analysis.
The SV Total RNA Isolation System provides a fast and simple technique for
preparing purified and intact total RNA from tissues, cultured cells
and white blood cells in as little as one hour, depending on the number of
samples to be processed. Like the Wizard® Plus SV DNA
Purification System (Cat.# A1330), either a spin or vacuum ("SV")
purification protocol can be used. Up to 60mg of tissue can be processed per
purification, depending on the type, function and RNA expression levels of the
tissue. The system also incorporates a DNase treatment step that is designed to
substantially reduce genomic DNA contamination, which can interfere with
amplification-based methodologies. Purification is achieved without the use of
phenol:chloroform extractions or ethanol precipitations, and there is no DNase
carryover in the final RNA preparation. |
