Abstract for the GeneEditor™ in vitro Site-Directed Mutagenesis System

Part# TM047
Printed in USA. Revised 4/09.
Instructions for Use of Product Q9280.

The GeneEditor™ in vitro Site-Directed Mutagenesis System is a high-efficiency system for the generation and selection of oligonucleotide-directed mutations. This system uses antibiotic selection to obtain a high frequency of mutants. Selection Oligonucleotides provided with the GeneEditor™ System encode mutations that alter the ampicillin resistance gene, creating a new additional resistance to the GeneEditor™ Antibiotic Selection Mix. In the GeneEditor™ System protocol, the Selection Oligonucleotide is annealed to a single- or double-stranded DNA template at the same time as a mutagenic oligonucleotide. Subsequent synthesis and ligation of the mutant strand links the two oligonucleotides. The resistance to the GeneEditor™ Antibiotic Selection Mix encoded by this mutant DNA strand facilitates selection of the desired mutation. The system will work with any cloning vector that contains ampicillin resistance as a selectable marker. Mutants generated using this system retain ampicillin resistance and gain resistance to the GeneEditor™ Antibiotic Selection Mix.