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Abstract for pCAT®3 Reporter Vectors
The pCAT®3 Reporter Vectors provide a basis for the quantitative
analysis of factors that potentially regulate mammalian gene expression. These
factors may be cis-acting, such as promoters and enhancers, or trans-acting,
such as various DNA-binding factors. The backbone of the pCAT®3
Reporter Vectors is similar to the pGL3 Luciferase Vectors with the exception of
an intron located 5´ of the chloramphenicol acetyltransferase (CAT) gene. The
vector backbones were designed to increase expression of the reporter gene for
easier monitoring of transcriptional activity in transfected eukaryotic cells.
In addition, the pCAT®3 Reporter Vectors contain numerous features
that aid in the structural characterization of the putative regulatory sequences.
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