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Abstract for PinPoint™ Xa Protein Purification System
The PinPoint™ Xa Protein Purification System is designed for the production
and purification of fusion proteins that are biotinylated in vivo. Biotinylated
fusion proteins are produced in E. coli and are affinity-purified using
the SoftLink™ Soft Release Avidin Resin. This proprietary resin allows elution
of the fusion protein under nondenaturing conditions. The PinPoint™ Vectors
feature the encoded endoproteinase Factor Xa (pronounced “ten a”)
proteolytic site that provides a way to separate the purification tag from the
native protein. These vectors also carry a convenient multiple cloning region
for ease in construction of fusion proteins. The system contains vectors in all
possible sense reading frames, an avidin-conjugated resin, Streptavidin-Alkaline Phosphatase, a purification column and biotin. The PinPoint™ Xa Control Vector
contains the chloramphenicol acetyltransferase (CAT) gene and is provided as a
means of monitoring protein expression, purification and processing conditions.
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