Abstract for PolyATtract® mRNA Isolation Systems
The PolyATtract® mRNA Isolation Systems utilize the
MagneSphere® technology to eliminate the need for oligo(dT)
cellulose and its associated problems. With total RNA as the starting material,
the poly(A) mRNA fraction can be isolated free of other nucleic acid contamination in approximately 45 minutes. The isolated mRNA is suitable for all
molecular biology applications, including in vitro translation and cDNA
synthesis.
The system uses a biotinylated oligo(dT) primer to hybridize at high
efficiency in solution to the 3′ poly(A) region present in most mature
eukaryotic mRNA species. The hybrids are captured and washed at high stringency
using streptavidin coupled to paramagnetic particles and a magnetic separation
stand. The mRNA is eluted from the solid phase by the simple addition of
ribonuclease-free deionized water. This procedure yields an essentially pure
fraction of mature mRNA after only a single round of magnetic separation.
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