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Abstract for pGL2 Luciferase Reporter Vectors
The pGL2 Luciferase Reporter Vectors provide a basis for the quantitative analysis
of factors that potentially regulate mammalian gene expression. These factors may
be cis-acting, such as promoters and enhancers, or trans-acting, such as various
DNA-binding factors. The pGL2 Vectors carry the coding region for firefly (Photinus
pyralis) luciferase, used to monitor transcriptional activity in transfected eukaryotic
cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition,
the pGL2 Vectors contain numerous features that aid the characterization and
mutagenesis of the putative regulatory sequences.
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