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Abstract for PowerPlex® Matrix Standards, 310
Proper generation of a matrix file is critical to evaluate
multicolor systems with the ABI PRISM® 310 Genetic Analyzer and the
ABI PRISM® 377 DNA Sequencer. To prepare a matrix, four standards
are run under the same capillary electrophoresis (CE) or gel
conditions that are used for samples and allelic ladders. The
PowerPlex® Matrix Standards, 310, consist of DNA fragments labeled
with four different fluorescent dyes: one tube contains DNA
fragments labeled with fluorescein, one tube contains DNA fragments
labeled with carboxy-tetramethylrhodamine (TMR), one tube contain
DNA fragments labeled with
6-carboxy-4´,5´-dichloro-2´,7´-dimethoxyfluorescein (JOE), and one
tube contains DNA fragments labeled with carboxy-X-rhodamine (CXR).
Use the fluorescein Matrix, JOE Matrix, TMR Matrix and CXR Matrix
for the blue, green, yellow and red standards, respectively.
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