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Abstract for Differex™ System
The Differex™ System uses the standard proteinase K digestion
and a combination of phase separation and differential centrifugation
for the separation of sperm and epithelial DNA. After the proteinase K
digestion, the sample and buffer are placed in a spin basket seated in
a tube containing a nonaqueous, nontoxic, biodegradable Separation
Solution. This solution is denser than, and nonmiscible with, the aqueous
buffer but is less dense than sperm. During centrifugation, the sperm
pellets to the bottom of the tube. The aqueous solution containing the
epithelial DNA remains on top of the nonaqueous solution. Removal of
the aqueous solution is aided by the yellow color of the buffer. Only
a thin film of the aqueous solution usually remains. This contaminating
solution is easily removed by adding water to dilute the remaining DNA
solution. The water is not miscible with the bottom solution and can be
easily removed without additional centrifugation.
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