Abstract for PowerPlex^® Matrix Standards, 310/377 Technical Bulletin

Part# TBD018
Printed in USA. Revised 8/09.
Instructions for Use of Product DG3640.

Proper generation of a matrix file is critical to evaluate multicolor systems with the ABI PRISM^® 310 Genetic Analyzer and the ABI PRISM^® 377 DNA Sequencer. The PowerPlex^® Matrix Standards, 310/377 was developed for use with the PowerPlex^® 16 System. It can also be used with the PowerPlex^® 1.2 System (fluorescein and TMR-labeled) or any of the GenePrint^® Fluorescent STR Systems (fluorescein-labeled).

To prepare a matrix, four standards are run under the same capillary electrophoresis (CE) or gel conditions that are used for samples and allelic ladders. The PowerPlex^® Matrix Standards, 310/377 consists of DNA fragments labeled with four different fluorescent dyes: one tube contains DNA fragments labeled with fluorescein, one tube contains DNA fragments labeled with carboxy-tetramethylrhodamine (TMR), two tubes contain DNA fragments labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy -fluorescein (JOE), and one tube contains DNA fragments labeled with carboxy-X-rhodamine (CXR). A matrix should be generated for each individual instrument.