|
Download
protocol
194kb
pdf? |
|
Abstract for PowerPlex® Matrix Standards,
310/377
Proper generation of a matrix file is critical to evaluate multicolor systems
with the ABI PRISM® 310 Genetic Analyzer and the ABI PRISM®
377 DNA Sequencer. The PowerPlex® Matrix Standards, 310/377 was developed for use with the
PowerPlex® 16 System. It can also be used with the PowerPlex®
1.2 System (fluorescein and TMR-labeled) or any of the GenePrint®
Fluorescent STR Systems (fluorescein-labeled).
To prepare a matrix, four standards are run under the same capillary
electrophoresis (CE) or gel conditions that are used for samples and allelic
ladders. The PowerPlex® Matrix Standards, 310/377 consists of DNA fragments labeled with four
different fluorescent dyes: one tube contains DNA fragments labeled with
fluorescein, one tube contains DNA fragments labeled with
carboxy-tetramethylrhodamine (TMR), two tubes contain DNA fragments labeled with
6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy -fluorescein (JOE), and one tube
contains DNA fragments labeled with carboxy-X-rhodamine (CXR). A matrix should
be generated for each individual instrument.
|
