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Abstract for SAM2® Biotin Capture Membrane
The SAM2® Biotin Capture Membrane binds biotinylated molecules
based on their affinity for streptavidin. The proprietary process by which the
SAM2® Membrane is produced results in a high density of streptavidin
on the filter, providing rapid, quantitative substrate binding in the nmol/cm2
range. In addition, the membrane has been optimized for low nonspecific binding.
The membrane is available either as a large, prenumbered, partially cut sheet
(approximately 10.5 x 15.0cm; Cat.# V2861) or as a smaller, uncut sheet
(approximately 7.6 x 10.9cm; Cat.# V7861). The partially cut membrane (Cat.#
V2861) allows easy separation into 96 individual squares and is designed for
small-scale experiments where high binding capacity is required. The uncut sheet
(Cat.# V7861) can be analyzed as a whole membrane or may be cut into the size
desired. The uncut membrane allows for sample application using a multichannel
pipettor. Both membranes may be analyzed using phosphorimaging, autoradiography
or scintillation counting to quantitate results. The membranes have also been
used successfully with chemiluminescence detection techniques. The use of
fluorescence for detection of captured molecules is not recommended at this
time. The SAM2® Membrane, used as recommended in this technical
bulletin, provides a number of advantages over other commercially available
streptavidin products.
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