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Abstract for T4 Polynucleotide Kinase
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the γ-phosphate
from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a
3´-phosphate group. T4 PNK is widely used to end-label short oligonucleotide
probes, DNA and RNA molecules. Under certain conditions, the reaction with
polynucleotides can be made reversible, permitting exchange of the γ-phosphate
of ATP with the 5´ terminal phosphate of a polynucleotide, thus circumventing
the need to dephosphorylate the substrate with alkaline phosphatase.
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