Abstract for Kinase-Glo^® Luminescent Kinase Assay Platform

Part# TB372
Printed in USA. Revised 5/09.
Instructions for Use of Products V6711, V6712, V6713, V6714, V3771, V3772, V3773, V3774, V6071, V6072, V6073, & V6074.

The Kinase-Glo^® Luminescent Kinase Assay Platform provides a homogeneous, high-throughput screening method for measuring kinase activity by quantitating the amount of ATP remaining in solution following a kinase reaction. The Kinase-Glo^® Platform consists of three assay formats: the Kinase-Glo^® Assay, which is used to monitor kinase activity using up to 10µM ATP; the Kinase-Glo^® Plus Assay, which is used for assays requiring higher ATP concentrations (up to 100µM); and the Kinase-Glo^® Max Assay, which is used for assays requiring up to 500µM ATP. The Kinase-Glo^® Assays are performed in a single well of a multiwell plate by adding a volume of Kinase-Glo^® Reagent equal to the volume of a completed kinase reaction and measuring luminescence. The luminescent signal is correlated with the amount of ATP present and is inversely correlated with the amount of kinase activity.