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Abstract for CellTiter-Fluor™ Cell Viability Assay
The CellTiter-Fluor™ Cell Viability Assay is a nonlytic, single-reagent addition
fluorescence assay that measures the relative number of live cells
in a
culture population after experimental manipulation. The
CellTiter-Fluor™ Cell Viability Assay measures a conserved and
constitutive
protease activity within live cells and therefore serves as a marker
of cell
viability. Results obtained using the CellTiter-Fluor™ Cell
Viability Assay
correlate well with other established methods of determining cell
viability. The live-cell protease activity is restricted to intact
viable cells and is
measured using a fluorogenic, cell-permeant, peptide substrate (glycylphenylalanyl-
aminofluorocoumarin; GF-AFC). The substrate enters intact cells
where it is cleaved by the live-cell protease activity to generate a
fluorescent
signal proportional to the number of living cells. This live-cell
protease becomes inactive upon loss of cell membrane integrity and
leakage
into the surrounding culture medium.
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