Abstract for Caspase-Glo^® 6 Assay

Part# TB366
Printed in USA. Revised 5/09.
Instructions for Use of Products G0970 & G0971.

The Caspase-Glo^® 6 Assay is a homogeneous, luminescent assay that measures caspase-6 activity.The Caspase-Glo^® 6 Assay provides a luminogenic caspase-6 substrate, Z-VEID-aminoluciferin, in a buffer system optimized for caspase-6 and luciferase activities. The addition of a single Caspase-Glo^® 6 Reagent in an “add-mix-measure” format results in caspase-6 cleavage of the substrate and generation of a glow-type luminescent signal produced by the luciferase reaction. In this homogeneous, coupled-enzyme format, the luminescent signal is proportional to the amount of caspase-6 activity present. The Caspase-Glo^® 6 Reagent relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase) that is formulated to generate a stable glow-type luminescent signal across a wide range of assay conditions.