Abstract for Caspase-Glo^® 2 Assay

Part# TB365
Printed in USA. Revised 5/09.
Instructions for Use of Products G0940 & G0941.

The Caspase-Glo^® 2 Assay is a homogeneous, luminescent assay that measures caspase-2 activity.The Caspase-Glo^® 2 Assay provides a luminogenic caspase-2 substrate, Z-VDVAD-aminoluciferin, in a reagent optimized for caspase-2 and luciferase activity. The VDVAD pentapeptide sequence is an optimized recognition sequence for caspase-2, although this sequence is also an acceptable substrate for caspases-3 and -7. A single Caspase-Glo^® 2 Reagent is added to test samples, resulting in caspase cleavage of the substrate and generation of a glow-type luminescent signal produced by luciferase. Luminescence is proportional to the amount of caspase activity present.