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Abstract for Caspase-Glo® 2 Assay
The Caspase-Glo® 2 Assay is a homogeneous, luminescent assay that measures caspase-2 activity.The Caspase-Glo® 2 Assay provides a
luminogenic caspase-2 substrate, Z-VDVAD-aminoluciferin, in a reagent
optimized for caspase-2 and luciferase activity. The VDVAD pentapeptide sequence is an optimized
recognition sequence for caspase-2, although this sequence is also an acceptable
substrate for caspases-3 and -7. A single Caspase-Glo® 2 Reagent is added to
test samples, resulting in caspase cleavage of the substrate and
generation of a glow-type luminescent signal produced by luciferase. Luminescence is proportional to the amount of caspase activity present.
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