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Abstract for CytoTox-Glo™ Cytotoxicity Assay
The CytoTox-Glo™ Cytotoxicity
Assay uses a luminogenic peptide substrate, the AAF-Glo™ Substrate,
to measure dead-cell protease activity, which is released from cells that
have lost membrane integrity. The AAF-Glo™ Substrate cannot cross the
intact membrane of live cells and does not generate any appreciable signal
from the live-cell population. The CytoTox-Glo™ Assay relies on the
properties of Ultra-Glo™ Recombinant Luciferase, which uses aminoluciferin
as a substrate to generate a stable "glow-type" luminescent signal and
is formulated to improve performance across a wide range of assay conditions. |
