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Abstract for cAMP-Glo™ Assay
The cAMP-Glo™ Assay is a
homogeneous, bioluminescent and high-throughput assay to measure
cAMP levels in cells. The cAMP-Glo™ Assay monitors cAMP production
in cells in response to the effects of an agonist or test compound on
G protein-coupled receptors (GPCR). GPCRs that couple with adenylate
cyclase will increase or decrease intracellular cAMP.
The assay is based on the principle that cyclic AMP (cAMP)
stimulates protein kinase A (PKA) holoenzyme activity, decreasing
available ATP and leading to decreased light production in a coupled
luciferase reaction. |
