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Abstract for CytoTox-Fluor™ Cytotoxicity Assay
The CytoTox-Fluor™ Cytotoxicity Assay is a
single-reagent-addition, homogeneous, fluorescent assay that
measures the relative number of dead cells in cell populations. The
CytoTox-Fluor™ Assay measures a distinct protease activity
associated with cytotoxicity. The assay uses a fluorogenic peptide
substrate (bis-alanyl-alanyl-phenylalanyl-rhodamine 110;
bis-AAF-R110) to measure dead-cell protease activity, which has been
released from cells that have lost membrane integrity. The
bis-AAF-R110 Substrate cannot cross the intact membrane of live
cells and therefore gives no signal from live cells.
The CytoTox-Fluor™ Assay is designed to
accommodate downstream multiplexing with many Promega luminescent
assays or spectrally distinct fluorescent assay methods, such as
assays measuring caspase activation, reporter expression or
orthogonal measures of viability. |
